Pee-wee Herman's second Marvel Comics appearance came on June 13, 1989, where he appeared alongside several other real life celebrities in "Avengers Spotlight" Vol. 1 #23. Paul Reubens appears during the second story of the comic, "Second Debut," in which the stoic android Vision takes a trip to Hollywood.
At one point in the story Vision finds himself on "The Tonight Show Starring Johnny Carson," and he sits in the interview chair next to Paul Reubens (dressed in costume as Pee-wee Herman) and Charo. During the interview Reubens mentions the blue-faced genie Jambi (John Paragon) from "Pee-wee's Playhouse," whom Carson refers to as "the poor man's Doctor Strange." The interview ends abruptly when Vision passes through the floor and departs Hollywood, leaving Reubens and Charo alone on the couch.
From there we cut to Avengers headquarters, where Captain America, Ant-Man, the Scarlet Witch, Tigra, and several other superheroes are laughing at Vision's interview, being broadcast shortly after his departure. Though Reubens is technically not in character for this appearance, the fact that he's wearing Pee-wee's iconic outfit is much more on the nose than his parody appearance in the "Incredible Hulk." In any case, the fact that the character was featured in two separate Marvel Comics stories ought to tell you just how popular Pee-wee Herman really was during the '80s.
